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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production

Fig. 1

Expression of n-butanol biosynthesis genes. a Scheme of a constructed n-butanol biosynthesis route in Synechocystis, with a competing PHB synthesis pathway deleted. Green arrows represent native genes, while blue, black and red arrows represent heterologously expressed genes. b Western blot of butanol biosynthesis enzymes from strain JA02 (phaJ ter adhE2 under P psbA2 ) and JA04 (phaJ ter adhE2 under P trc ) at nitrogen replete (N+) and deplete (N−) conditions. 12 μg total protein was loaded from each sample

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