Table 1 Plasmids and strains used in the present study
From: Bacterial synthesis of N-hydroxycinnamoyl phenethylamines and tyramines
Plasmids or E. coli strain | Relevant properties or genetic marker(s) | Source or references |
|---|---|---|
Plasmids | ||
pACYCDuet | P15A ori, Cmr | Novagen |
pCDFDuet | CloDE13 ori, Strr | Novagen |
pETDuet | f1 ori, Ampr | Novagen |
pA-SeTAL | pACYCDuet carrying TAL from Saccharothrix espanaensis | [31] |
pA-aroG-SeTAL-tyrA | pACYCDuet carrying TAL from S. espanaensis, aroG and tyrA from Escherichia coli | [31] |
pA-aorGfbr-SeTAL-tyrAfbr | pACYCDuet carrying TAL from S. espanaensis, aroG fbr, and tyrA fbr from E. coli | |
pE-PDC | pETDuet carrying PDC from Pseudomonas putida | |
pC-Os4CL-SHT | pCDFDuet carrying 4CL from Oryza sativa and SHT from Capsicum annuum | |
pC-Os4CL-THT | pCDFDuet carrying 4CL from O. sativa and THT from C. annuum | This study |
pC-TDC-Os4CL-THT | pCDFDuet carrying TDC from Papaver somniferum, 4CL from O. sativa, and THT from C. annuum | This study |
Strains | ||
BL21 (DE3) | F− ompT hsdS B (r −B m −B ) gal dcm lon (DE3) | Novagen |
BtyrR | BL21(DE3) ΔtyrR | [31] |
BtyrR-trpD | BL21(DE3) ΔtyrR/ΔtrpD | This study |
HP-1 | BL21 harboring pC-Os4CL-SHT | This study |
HP-2 | BL21 harboring pC-Os4CL-SHT and pE-PDC | This study |
HP-3 | BL21 harboring pC-Os4CL-SHT, pA-SeTAL, and pE-PDC | This study |
HP-4 | BL21 harboring pC-Os4CL-SHT, pA-aroG-SeTAL-tyrA, and pE-PDC | This study |
HP-5 | BL21 harboring pC-Os4CL-SHT, pA-aroGfbr-SeTAL-tyrAfbr, and pE-PDC | This study |
HP-6 | BtyrR harboring pC-Os4CL-SHT, pA-aroGfbr-SeTAL-tyrAfbr, and pE-PDC | This study |
HP-7 | BtyrR-trpD harboring pC-Os4CL-SHT, pA-aroGfbr-SeTAL-tyrAfbr, and pE-PDC | This study |
HT-1 | BL21 harboring pC-Os4CL-THT | This study |
HT-2 | BL21 harboring pC-TDC-Os4CL-THT | This study |
HT-3 | BL21 harboring pC-TDC-Os4CL-THT and pA-aroG-tyrA | This study |
HT-4 | BL21 harboring pC-TDC-Os4CL-THT and pA-aroGfbr-tyrAfbr | This study |
HT-5 | BL21 harboring pC-TDC-Os4CL-THT and pA-SeTAL | This study |
HT-6 | BL21 harboring pC-TDC-Os4CL-THT and pA-aroG-SeTAL-tyrA | This study |
HT-7 | BL21 harboring pC-TDC-Os4CL-THT and pA-aroGfbr-SeTAL-tyrAfbr | This study |
HT-8 | BtyrR-pheA harboring pC-TDC-Os4CL-THT and pA-aroGfbr-SeTAL-tyrAfbr | This study |
HT-9 | BtyrR-trpD harboring pC-TDC-Os4CL-THT and pA-aroGfbr-SeTAL-tyrAfbr | This study |