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Table 1 Strains and plasmids used in this work

From: Genome-wide identification and evaluation of constitutive promoters in streptomycetes

Name

Description

Source

E. coli

 JM109

General cloning host for plasmid manipulation

Novagen

 ET12567/PUZ8002

Donor strain for conjugation between E. coli and Streptomyces

[44]

S. coelicolor

 M145

The model organism of Streptomyces

[44]

 M1146

Derivative of M145, completely lacks the biosynthetic clusters of Act, Red, CDA and yCPK

[31]

S. venezuelae

 ISP5230

Wild-type strain for jadomycin B production

[49]

 WVR2006

Jadomycin biosynthetic gene cluster deletion mutant

[32]

S. albus J1074

S. albus G1 (DSM 41398) derivative with the defective SalGI restriction modification system heterologous host

[33]

Plasmids

 pIJ8660

Containing the reporter gene gfp between the major transcription terminator of phage tfd and to

[42]

 pDR4-E

Containing ermE* promoter

[12]

 pIMermE*

Insert ermE*p into pIJ8660 to control the expression of gfp

This work

 pIM4503

Replace ermE*p and RBS of pIMermE* with Psco4503

This work

 pIM4508

Replace ermE*p and RBS of pIMermE* with Psco4508

This work

 pIM6740

Replace ermE*p and RBS of pIMermE* with Psco6740

This work

 pIM3410

Replace ermE*p and RBS of pIMermE* with Psco3410

This work

 pIM4658

Replace ermE*p and RBS of pIMermE* with Psco4658

This work

 pIM2468

Replace ermE*p and RBS of pIMermE* with Psco2468

This work

 pIM2074

Replace ermE*p and RBS of pIMermE* with Psco2074

This work

 pIM5768

Replace ermE*p and RBS of pIMermE* with Psco5768

This work

 pLN0

Remove the elements for site-specific recombination of pIMermE*

This work

 pLNermE*

Insert 5′ end of jadJ gene into pLN0 under the control of ermE*p

This work

 pLNsco6740p

Replace ermE*p and RBS of pLNermE* with Psco6740

This work

 pLNsco5768p

Replace ermE*p and RBS of pLNermE* with Psco5768

This work

 pLNsco2074p

Replace ermE*p and RBS of pLNermE* with Psco2074

This work

 pLNsco2468p

Replace ermE*p and RBS of pLNermE* with Psco2468

This work