From: Genome-wide identification and evaluation of constitutive promoters in streptomycetes
Name | Description | Source |
---|---|---|
E. coli | ||
 JM109 | General cloning host for plasmid manipulation | Novagen |
 ET12567/PUZ8002 | Donor strain for conjugation between E. coli and Streptomyces | [44] |
S. coelicolor | ||
 M145 | The model organism of Streptomyces | [44] |
 M1146 | Derivative of M145, completely lacks the biosynthetic clusters of Act, Red, CDA and yCPK | [31] |
S. venezuelae | ||
 ISP5230 | Wild-type strain for jadomycin B production | [49] |
 WVR2006 | Jadomycin biosynthetic gene cluster deletion mutant | [32] |
S. albus J1074 | S. albus G1 (DSM 41398) derivative with the defective SalGI restriction modification system heterologous host | [33] |
Plasmids | ||
 pIJ8660 | Containing the reporter gene gfp between the major transcription terminator of phage tfd and to | [42] |
 pDR4-E | Containing ermE* promoter | [12] |
 pIMermE* | Insert ermE*p into pIJ8660 to control the expression of gfp | This work |
 pIM4503 | Replace ermE*p and RBS of pIMermE* with Psco4503 | This work |
 pIM4508 | Replace ermE*p and RBS of pIMermE* with Psco4508 | This work |
 pIM6740 | Replace ermE*p and RBS of pIMermE* with Psco6740 | This work |
 pIM3410 | Replace ermE*p and RBS of pIMermE* with Psco3410 | This work |
 pIM4658 | Replace ermE*p and RBS of pIMermE* with Psco4658 | This work |
 pIM2468 | Replace ermE*p and RBS of pIMermE* with Psco2468 | This work |
 pIM2074 | Replace ermE*p and RBS of pIMermE* with Psco2074 | This work |
 pIM5768 | Replace ermE*p and RBS of pIMermE* with Psco5768 | This work |
 pLN0 | Remove the elements for site-specific recombination of pIMermE* | This work |
 pLNermE* | Insert 5′ end of jadJ gene into pLN0 under the control of ermE*p | This work |
 pLNsco6740p | Replace ermE*p and RBS of pLNermE* with Psco6740 | This work |
 pLNsco5768p | Replace ermE*p and RBS of pLNermE* with Psco5768 | This work |
 pLNsco2074p | Replace ermE*p and RBS of pLNermE* with Psco2074 | This work |
 pLNsco2468p | Replace ermE*p and RBS of pLNermE* with Psco2468 | This work |