Fig. 5

Product formation and sum of relative transcript abundances of four E. coli BL21(DE3) clones grown under inducing conditions. Soluble and total product formation (determined using a BioLector device or densitometric analysis, respectively) and sum of relative transcript abundances (determined from conventional shake flasks) as function of time for four E. coli BL21(DE3) clones belonging to respiration behavior Type A [Arg107-CGT (WT), -CGA; white background] or respiration behavior Type B (Arg107-CGC, -CGG; grey background) during cultivation in Wilms-MOPS mineral autoinduction medium containing 0.5 g/L glucose, 5 g/L glycerol, 2 g/L lactose. ‘lipA vs. cysG’ represents the sum of the relative transcript abundance of the plasmid-encoded target gene lipA (encoding Bacillus subtilis lipase A) in relation to the genome-encoded reference gene cysG (encoding uroporphyrin III C-methyltransferase). ‘bla vs. cysG’ represents the relative plasmid copy number as the sum of the relative transcript abundance plasmid-encoded reference gene bla (encoding beta-lactamase) in relation to the genome-encoded reference gene cysG. ‘lipA vs. bla’ represents the factor of lipA induction as transcript abundance of the plasmid-encoded target gene lipA in relation to the plasmid-encoded reference gene bla. The grey dotted lines separate the five cultivation phases presented in Fig. 4. Cultivation conditions: 37 °C, 250 mL flasks, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm (in conventional flasks); 37 °C, 48-well Flowerplate, filling volume 1 mL, shaking frequency 1000 rpm, shaking diameter 3 mm (in BioLector)