Table 1 Optical signals and applied setup for BioLector online monitoring
Optical signal | λex (nm) | λem (nm) | Integration time (ms) | Slit (nm) |
|---|---|---|---|---|
Biomass (scattered light) | 650 | – | 900 | 6 |
DOT | 620 | 760 | 128 | – |
GFP fluorescence | 488 | 520 | 600 | 8 |
YFP fluorescence | 510 | 532 | 600 | 8 |
mCherry fluorescence | 580 | 610 | 600 | 8 |