Fig. 7
Comparison of E. coli BL21 (DE3) pRotHi-YFP cultivations using the BioLector microtiter plate and the RAMOS shake flask. Online monitoring of the oxygen transfer rate (OTR) in a RAMOS shake flask and dissolved oxygen tension (DOT) via dispersed oxygen-sensitive nanoparticles in a MTP (a) and microbial growth via scattered light in a microtiter plate in the BioLector system (b). c Fluorescence intensity of YFP (ex.: 510 nm/em.: 532 nm). Cultivation medium: Synthetic Wilms-MOPS medium with 20 g L−1 glucose and 1.5 g L−1 sorbitol. Cultivation conditions: BioLector: 48well Round Well Plate without optodes, VL = 800 µL, n = 1000 rpm, d0 = 3 mm, 37 °C; RAMOS: 250 mL RAMOS shake flask, VL = 22 mL, n = 350 rpm, d0 = 50 mm, 37 °C. Based on the DOT measured in MTP the OTR was calculated with a fitted kLa-value of 211 h−1 according to Eq. 2. The data of the RAMOS cultivation is shifted 1.5 h. For a better comparison of the propagation of the scattered light signals, two specifically adjusted y axes were used