Fig. 6

Comparison of E. coli BL21 (DE3) pRSet-mCherry cultivations using the BioLector microtiter plate and the RAMOS shake flask system. Online monitoring of the oxygen transfer rate (OTR) in a RAMOS shake flask and dissolved oxygen tension (DOT) via dispersed oxygen-sensitive nanoparticles in a MTP (a) and microbial growth via scattered light in a microtiter plate in the BioLector system (b). c Fluorescence intensity of mCherry (ex.: 580 nm/em.: 610 nm). Cultivation medium: Synthetic Wilms-MOPS auto-induction medium with 0.55 g L⁻¹ glucose, 2 g L⁻¹ lactose and 5 g L⁻¹ glycerol. Cultivation conditions: BioLector: 48well Round Well Plate without optodes, V_L = 900 µL, n = 1000 rpm, d₀ = 3 mm, 37 °C; RAMOS: 250 mL RAMOS shake flask, V_L = 23 mL, n = 350 rpm, d₀ = 50 mm, 37 °C. Based on the DOT measured the OTR was calculated with a fitted k_La-value of 181 h⁻¹ according to Eq. 2. The data of the RAMOS cultivation is shifted 1.1 h. For a better comparison of the propagation of the scattered light signals, two specifically adjusted y axes were used