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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Online monitoring of dissolved oxygen tension in microtiter plates based on infrared fluorescent oxygen-sensitive nanoparticles

Fig. 5

Comparison of H. polymorpha RB11 PFMD-GFP cultivations using the BioLector microtiter plate and the RAMOS shake flask system. Online monitoring of the oxygen transfer rate (OTR) in a RAMOS shake flask and dissolved oxygen tension (DOT) via dispersed oxygen-sensitive nanoparticles in a MTP (a) and microbial growth via scattered light in a microtiter plate in the BioLector system (b). c Fluorescence intensity of GFP (ex.: 490 nm/em.: 510 nm). Cultivation conditions: BioLector: 48well Round Well Plate without optodes, VL = 800 µL, n = 1000 rpm, d0 = 3 mm, 30 °C; RAMOS: 250 mL-RAMOS shake flask, VL = 10 mL, n = 350 rpm, d0 = 50 mm, 30 °C; synthetic Syn-6-MES medium with 10 g L−1 glycerol. Based on the DOT measured in MTP the OTR was calculated with a fitted kLa-value of 188 h−1 according to Eq. 2. The data of the RAMOS cultivation was shifted –1.5 h. For a better comparison of the propagation of the scattered light signals, two specifically adjusted y axes were used

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