Fig. 3
Influence of autoclaving and the impact of the shaking frequency on the DOT measured inside a microtiter plate via dispersed oxygen-sensitive nanoparticles. Influence of autoclaving (121 °C, 21 min) (a). Impact of the shaking frequency on the signal intensity and on the phase angle as raw signal for the DOT measurement via non-autoclaved (b) and autoclaved dispersed oxygen-sensitive nanoparticles (c). Measuring conditions: synthetic Wilms-MOPS medium without glucose and with 1 g L−1 dispersed oxygen-sensitive nanoparticles, 48well round well MTP without optodes, VL = 800 µL, d0 = 3 mm, 30 °C; n varies and is given in the figure