Fig. 2

Biocompatibility of dispersed oxygen-sensitive nanoparticles. G. oxydans Δupp, E. coli BL21 (De3) pRSet-mCherry and H. polymorpha RB11 (P_FMD-GFP) were cultivated in the RAMOS device to determine the oxygen transfer rates (OTR) with and without 1 g L⁻¹ dispersed oxygen-sensitive nanoparticles. Cultivation conditions: G. oxydans: complex mannitol medium, 30 °C; E. coli: synthetic Wilms-MOPS auto-induction medium with 0.55 g L⁻¹ glucose, 2 g L⁻¹ lactose and 5 g L⁻¹ glycerol, 37 °C; H. polymorpha: synthetic Syn-6-MES medium with 10 g L⁻¹ glycerol, 30 °C; Shaking conditions: V_L = 10 mL in 250 mL RAMOS shake flask, n = 350 rpm, d₀ = 50 mm