Skip to main content

Table 1 Bacteriocin production and antimicrobial activity of supernatants from recombinant strains

From: Cloning strategies for heterologous expression of the bacteriocin enterocin A by Lactobacillus sakei Lb790, Lb. plantarum NC8 and Lb. casei CECT475

Strain

Bacteriocin production (µg/mg cell dry weight)a

Antimicrobial activity (BU/mg cell dry weight)b

Specific antimicrobial activity (BU/µg EntA)c

Lactobacillus sakei

 Lb790

NP

NA

NE

 Lb790 (pSIP409UAI)

1.3

324

249

 Lb790 (pSIP411UAI)

5.2

1578

303

 Lb790 (pMGUAI)

0.7

48

68

Lactobacillus plantarum

 NC8

NP

NA

NE

 NC8 (pSIP409UAI)

0.4

42

105

 NC8 (pSIP411UAI)

0.4

36

90

 NC8 (pMGUAI)

0.3

19

63

Lactobacillus casei

 CECT475

NP

102

NE

 CECT475 (pSIP409UAI)

1.7

958

1629

 CECT475 (pSIP411UAI)

9.3

16,466

1771

 CECT475 (pMGUAI)

1.1

869

790

Enterococcus faecium

 T136d

1.9

721

379

  1. Most of the data are mean from two independent determinations in triplicate
  2. NP no production, NA no activity, NE not evaluable
  3. aProduction of EntA was calculated by using a NCI-ELISA with polyclonal antibodies specific for EntA
  4. bAntimicrobial activity was calculated against E. faecium P13 (EntAs). BU, bacteriocin units
  5. cSpecific antimicrobial activity refers to the antimicrobial activity against E. faecium P13 divided by the EntA produced
  6. dCulture of E. faecium T136 used as control for production and antimicrobial activity of EntA