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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Prophage recombinases-mediated genome engineering in Lactobacillus plantarum

Fig. 2

Optimization of dsDNA recombination parameters. a The effect of the induction time on selection efficiency. The culture was incubated for 3–8 h before addition of the inducing peptides. 0.5 μg dsDNA substrates with 1.4-kb homologies were used for electroporation and the recovery time was 3 h. OD600 of the culture on induction is also shown. b The effect of the quantity of dsDNA substrates on selection efficiency. The culture was incubated for 4 h before addition of the inducing peptides. 0.5–5 μg dsDNA substrates with 1.4-kb homologies were used for electroporation and the recovery time was 3 h. c The effect of the recovery time on selection efficiency. The culture was incubated for 4 h before addition of the inducing peptides. 1 μg dsDNA substrates were used for electroporation and the recovery time was 1–5 h. d The effect of the homology length of dsDNA substrates on selection efficiency. PCR products with various homology lengths were generated by PCR and added at constant molarity. The culture was incubated for 4 h before addition of the inducing peptides. 1 μg dsDNA substrates was used for electroporation and the recovery time was 1 h. Results are the averages from at least three independent experiments, with standard deviations indicated by error bars

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