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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Activation of formate hydrogen-lyase via expression of uptake [NiFe]-hydrogenase in Escherichia coli BL21(DE3)

Fig. 3

Combinatorial expressions of hydrogenase subunits of H. marinus in BL21(DE3). a Construction of expression vectors. The constructed vectors (from top to bottom) are pTrcHoxG, pTrcHoxK, pTrcHoxGK, pTrcHoxGK*, and pTrcHoxK*, respectively. b Western blot analysis. Anti-His6 antibody was used. c H2 production. d FHL activation. H2 production from formate was measured after 18-h incubation. RBS ribosome binding site, His 6 hexahistidine tag sequence, SP sequence for signal peptide, HoxK* HoxK without signal peptide

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