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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enhancement of 3-hydroxypropionic acid production from glycerol by using a metabolic toggle switch

Fig. 1

Metabolic pathway involved in central metabolism and 3-HP production from glycerol. Dashed arrows represent conditionally repressed reactions. Bold arrows represent the introduced synthetic pathway for 3-HP production. The inset indicates the structure of MTS. When IPTG was absent, LacI repressed the transcription from PllacO1. In the presence of IPTG, TetR repressed the transcription from PltetO1. Metabolite abbreviations: DHAP dihydroxyacetone phosphate, GAP glyceraldehyde 3-phosphate, 1,3-BPG 1,3-bisphosphoglycerate, 3-HPA 3-hydroxypropionaldehyde, 3-HP 3-hydroxypropinic acid, 1,3-PDO 1,3-propanediol. Metabolic enzymes: GlpK glycerol kinase, GlpD glycerol-3-phosphate dehydrogenases, TpiA triosephosphate isomerase, GapA glyceraldehyde-3-phosphate dehydrogenase, DhaB glycerol dehydratase, GDR glycerol dehydratase reactivator, AraE α-ketoglutaric semialdehyde dehydrogenase, YqhD 1,3-propandiol oxidoreductase

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