Table 2 Oligonucleotides used in this study
From: Construction and development of an auto-regulatory gene expression system in Bacillus subtilis
Primers | Sequencesa |
|---|---|
P1 | CTCTTCCGCTTCCTCGCTCACTGACTCGC |
P2 | GCGGTATTTTCTCCTTACGCATCTGTGCGG |
P3 | GCGAGTCAGTGAGCGAGGAAGCGGAAGAGTAGAAGAAGCTTGGAGACAAGGTAAAGG |
P4 | CCGCACAGATGCGTAAGGAGAAAATACCGCCATATGTAAATCGCTCCTTTTTAGGTGGCAC |
P5 | CCCCCTTTGCTGAGGTGGCAGAGGGCAGGTATCGACAAAAATGTCATGAA |
P6 | CCGCACAGATGCGTAAGGAGAAAATACCGCCATTGTCATACCTCCCCTAATCTTTATAAG |
P7 | ACCTGCCCTCTGCCACCTCAGC |
P8 | GCGGTATTTTCTCCTTACGCATC |
P9 | CTTATAAAGATTAGGGGAGGTATGACAATGATGAGTAAAGGAGAAGAACTTTTCACTGGAG |
P10 | CCGCACAGATGCGTAAGGAGAAAATACCGCTTATTTGTATAGTTCATCCATG |
P11 | CATTGTCATACCTCCCCTAATC |
P12 | ATGAGTAAAGGAGAAGAACT |
P13 | ACTTTTCACCCATTTTTCGGTTGACAAAACATTTTTTTCATTTATAATGAACGGTAGAAAGATAAAAAATATTGAAA |
P14 | TTTTATCTTTCTACCGTTCATTATAAATGAAAAAAATGTTTTGTCAACCGAAAAATGGGTGAAAAGTTTCATGCGGG |
P15 | CTTATAAAGATTAGGGGAGGTATGACAATGATGAAAAAGCTTTTGACTGTC |
P16 | CCGCACAGATGCGTAAGGAGAAAATACCGCTTATTTGATATCTTCAAAAATG |
P17 | CGCAGATGTAGTCAACACCGAGTGCGTC |
P18 | ATCAAGAGTTTACATAATATATATTCTAGGAAGTATCAATCAATTCCATATAGCCTTTCCC |
P19 | ACTGAACTTCCTAGAATATATATTATGTAAACTATGGAAATAACTTTTTACCCTTTAACGG |
P20 | ATCGTTGATTAGGAGATTATACGG |
P21 | TTGATACTTCCTAGAATATATATTATGTAAACTCTTGATATGGCTTTTTATATGTG |
P22 | TCCATAGTTTACATAATATATATTCTAGGAAGTTCAGTCCTGCTCCTCGGCCACG |