Fig. 4From: Construction and development of an auto-regulatory gene expression system in Bacillus subtilis The expression level of GFP controlled by the mutant PsrfA (mutPsrfA) in B. subtilis 168. The recombinant strain harboring pBSG05 was constructed to analyze the alteration of the transcription and expression pattern. During 100 h of cultivation, cells were sampled periodically and analyzed by examining the cell density and fluorescence intensityBack to article page