Table 1 Number of charged residues, calculated/measured isoelectric point, and Tm for sdAb
From: Enhanced production of a single domain antibody with an engineered stabilizing extra disulfide bond
Clone | Tm (°C) Dye melt | Tm (°C) CD | Refolding (%) | Yield (mg/L)a |
|---|---|---|---|---|
L1-G2 | 64 | 62 | 55 | 24.6 ± 5.1 (3) |
L1-G2+ | 81 | 78 | 15 | 3.9 ± 1.1 (7) |
L1-G2+neg | 81 | 78 | 70 | 13.8 ± 1.4 (4) |
L1-G2+neg2 | 81 | 78 | 78 | 7.9 (2) |
A3 | 86 | 85 | 73 | ≥11.5 (4) |
A3+ | 87 | >90 | 94 | 1.7 ± 0.8 (4) |
A3+neg | 85 | 87 | 87 | 2.3 ± 0.7 (4) |
AC | 73 | 74 | 63 | 24.5 (2) |
ACneg | 80 | 81 | 87 | 24 (2) |
ACneg2 | 81 | 80 | 88 | 17 (2) |
AC+ | 76 | 82 | 69 | 3.2 ± 0.6 (6) |
AC+neg | 82 | 86 | 82 | 4.1 ± 0.5 (6) |
AC+neg2 | 81 | 85 | 84 | 4.2 ± 0.5 (6) |