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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enhanced production of a single domain antibody with an engineered stabilizing extra disulfide bond

Fig. 1

Sequence alignment using MultAlin [29] and CD heating and cooling curves. Top panel shows the sequence alignment of L1-G2, L1-G2+, L1-G2+neg, and L1-G2+neg2. The blue bars indicate the positions of CDR1, 2, and 3. The initial two amino acids (MA) and the amino acids added due to the restriction site and the His-tag are not show above (AAALEHHHHHH). The bottom panels show the melting and re-folding of the sdAb L1-G2, L1-G2+, L1G2+neg, L1G2+neg2 as measured by CD. Heating curves are shown in red and cooling in blue

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