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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Proteomic analysis of the influence of Cu2+ on the crystal protein production of Bacillus thuringiensis X022

Fig. 3

Real-time qRT-PCR analysis of selected genes. qRT-PCR was used to substantiate the differential expression patterns of 14 selected genes (cry1Ca, cry1Da, cry1Ac, phaR, BDH, EF-G, KAS II, ALDH, SHDA, ATPSβ, PrkA, IMPDH, SASPB, and OAT). mRNA levels after 31 h of culture in the original medium (CK) and medium to which Cu2+ had been added (Cu) were analyzed as values relative to the 16S rRNA gene. The ratio value for CK was set to 1. Error bars are calculated from three independent determinations of mRNA abundance in each sample

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