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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Designed biosynthesis of 25-methyl and 25-ethyl ivermectin with enhanced insecticidal activity by domain swap of avermectin polyketide synthase

Fig. 3

Gene replacement in S. avermitilis NA-108. a Schematic description of the gene replacement of aveDH2-KR2 in S. avermitilis NA-108 with milDH2-ER2-KR2 from S. bingchenggensis via double crossover. b PCR analysis with genomic DNA from S. avermitilis NA-108, S. avermitlis AVE-T27 and S. bingchenggensis, using primers E1 and E2. Lane 1 S. bingchenggensis; Lane 2 S. avermitilis AVE-T27; Lane 3 S. avermitilis NA-108; Lane 4 DNA ladder. c Schematic description of the gene replacement of aveLAT-ACP in S. avermitilis AVE-T27 with milLAT-ACP from S. bingchenggensis via double crossover. d PCR analysis with genomic DNA from S. avermitilis AVE-T27, S. avermitlis AVE-H39 and S. bingchenggensis, using primers V1 and V2. Lane 1 DNA ladder; Lane 2 S. bingchenggensis; Lane 3 S. avermitilis AVE-T27; Lane 4 S. avermitilis AVE-H39

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