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Table 2 Bacterial strains, plasmids and cosmids used and generated in this study

From: A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes

Strain/plasmid/cosmid Description Source of reference
Strain
 E. coli BT340 DH5α/pCP20 [45]
 E. coli BW25113 K-12 derivative: ΔaraBAD, ΔrhaBAD [46]
 E. coli DH5α For cloning and propagation of host strain, deoR recA1 endA1 hsdR17(rk-, mk +) phoA supE44 thi-1 gyrA96 relA1 λ Invitrogen, USA
 E. coli ET12567 dam-13::Tn9 dcm-6 hsdM hsdS [47]
 S. coelicolor M1152 Derivative of S. coelicolor M145, Δact Δred Δcpk Δcda rpoB(C1298T) [28]
 S. coelicolor M1314 S. coelicolor M1152 Δsco7221 This study
 S. coelicolor M1316 S. coelicolor M1152 Δsco7221 Δsco7669-7670-7671 This study
 S. coelicolor M1317 S. coelicolor M1152 Δsco7221 Δsco7669-7670-7671 Δsco1206-1207-1208::(oriT-aac(3)IV) This study
Plasmid
 pGEM®-T Easy E. coli vector for cloning PCR product, ampr Promega, USA
 pIJ86 ermEp*, aac(3)IV, oriT (RK2), ori (pIJ101), ori (pUC18) [48]
 pIJ773 pBluescript KS (+), aac(3)IV, oriT (RK2), FRT sites [35]
 pIJ790 λ-RED (gam, bet, exo), cat, araC, rep101 ts [35]
 pIJ10701 pBluescript KS (+), hyg, oriT (RK2), bla [35]
 pIJ12477 pIJ86, neo [49]
 pIJ13000 St2H12 ∆sco7221::(oriT-aac(3)IV) This study
 pIJ13001 St4C2 ∆sco7669-7670-7671::(oriT-aac(3)IV) This study
 pIJ13002 2StG58 ∆sco1206-1207-1208::(oriT-aac(3)IV) This study
 pIJ13003 St2H12 ∆sco7221 This study
 pIJ13004 pIJ13003::(oriT-hyg) This study
 pIJ13005 St4C2 ∆sco7669-7606-7671 This study
 pIJ13006 pIJ13005::(oriT-hyg) This study
 pIJ13007 2StG58 ∆sco1206-1207-1208 This study
 pIJ13008 pIJ13007::(oriT-hyg) This study
 pUZ8002 Kanr /tra, neo, RP4 [50]
Cosmid
 2StG58 SuperCos1 containing sco1206-1207-1208 John Innes Centre, UK
 St2H12 SuperCos1 containing sco7221 John Innes Centre, UK
 St4C2 SuperCos1 containing sco7669-7670-7671 John Innes Centre, UK
 Sv3G11 SuperCos1 containing sven5367 John Innes Centre, UK
 SuperCos1 neo, bla Agilent Technology, USA