Table 2 Bacterial strains, plasmids and cosmids used and generated in this study
From: A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes
Strain/plasmid/cosmid | Description | Source of reference |
|---|---|---|
Strain | ||
 E. coli BT340 | DH5α/pCP20 | [45] |
 E. coli BW25113 | K-12 derivative: ΔaraBAD, ΔrhaBAD | [46] |
 E. coli DH5α | For cloning and propagation of host strain, deoR recA1 endA1 hsdR17(rk-, mk +) phoA supE44 thi-1 gyrA96 relA1 λ − | Invitrogen, USA |
 E. coli ET12567 | dam-13::Tn9 dcm-6 hsdM hsdS | [47] |
 S. coelicolor M1152 | Derivative of S. coelicolor M145, Δact Δred Δcpk Δcda rpoB(C1298T) | [28] |
 S. coelicolor M1314 | S. coelicolor M1152 Δsco7221 | This study |
 S. coelicolor M1316 | S. coelicolor M1152 Δsco7221 Δsco7669-7670-7671 | This study |
 S. coelicolor M1317 | S. coelicolor M1152 Δsco7221 Δsco7669-7670-7671 Δsco1206-1207-1208::(oriT-aac(3)IV) | This study |
Plasmid | ||
 pGEM®-T Easy | E. coli vector for cloning PCR product, ampr | Promega, USA |
 pIJ86 | ermEp*, aac(3)IV, oriT (RK2), ori (pIJ101), ori (pUC18) | [48] |
 pIJ773 | pBluescript KS (+), aac(3)IV, oriT (RK2), FRT sites | [35] |
 pIJ790 | λ-RED (gam, bet, exo), cat, araC, rep101 ts | [35] |
 pIJ10701 | pBluescript KS (+), hyg, oriT (RK2), bla | [35] |
 pIJ12477 | pIJ86, neo | [49] |
 pIJ13000 | St2H12 ∆sco7221::(oriT-aac(3)IV) | This study |
 pIJ13001 | St4C2 ∆sco7669-7670-7671::(oriT-aac(3)IV) | This study |
 pIJ13002 | 2StG58 ∆sco1206-1207-1208::(oriT-aac(3)IV) | This study |
 pIJ13003 | St2H12 ∆sco7221 | This study |
 pIJ13004 | pIJ13003::(oriT-hyg) | This study |
 pIJ13005 | St4C2 ∆sco7669-7606-7671 | This study |
 pIJ13006 | pIJ13005::(oriT-hyg) | This study |
 pIJ13007 | 2StG58 ∆sco1206-1207-1208 | This study |
 pIJ13008 | pIJ13007::(oriT-hyg) | This study |
 pUZ8002 | Kanr /tra, neo, RP4 | [50] |
Cosmid | ||
 2StG58 | SuperCos1 containing sco1206-1207-1208 | John Innes Centre, UK |
 St2H12 | SuperCos1 containing sco7221 | John Innes Centre, UK |
 St4C2 | SuperCos1 containing sco7669-7670-7671 | John Innes Centre, UK |
 Sv3G11 | SuperCos1 containing sven5367 | John Innes Centre, UK |
 SuperCos1 | neo, bla | Agilent Technology, USA |