Fig. 5
From: A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes
Flaviolin production in derivatives of S. coelicolor M1152. a HPLC chromatogram of flaviolin (F) production in M1152/pIJ12477 + sven5367 and M1317/pIJ12477 + sven5367; in each case, a mass of [M + H]− 205 m/z was confirmed by mass spectrometry (data for M1317/pIJ12477 + sven5367 is shown in the inset). GA and GB7 indicate peaks of germicidin A and germicidin B7, respectively. The HPLC profiles for M1152 and M1317 were indistinguishable from those for M1152/pIJ86 and M1317/pIJ12477, respectively (data not shown). b Flaviolin production per mg dry biomass weight. The histograms show the average values obtained from duplicate cultures with the two individual measurements (Table 1) indicated by the bars