Microbial Cell Factories

Table 1 Vectors for the E. coli whole-cell system

From: A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol

Vector	Reductase	Origin	Ferredoxin	Origin
p21b_bRED	CPR	Bos taurus
p21b_AdAx	AdR	Bos taurus	Adx	Bos taurus
p21b_ArAx	arh1	S. pombe	Adx	Bos taurus
p21b_FrAx	Fpr	E. coli	Adx	Bos taurus
p21b_ArEt	arh1	S. pombe	etp1^fd	S. pombe
p21b_FrEt	Fpr	E. coli	etp1^fd	S. pombe

Six pET17b based vectors were constructed, each carrying the CYP21A2 cDNA, in either a bicistronic construct with the cDNA for CPR or in a tricistronic construct with the cDNAs for Adx _1–108 or etp1 ^fd as final electron donors. The origin of the respective protein is mentioned

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