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Fig. 8 | Microbial Cell Factories

Fig. 8

From: A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol

Fig. 8

Time-dependent premedrol formation by the CYP21A2 based whole-cell system with arh1 and etp1fd in a bioreactor. Whole-cell biotransformation in a bioreactor was performed with cells of C43(DE3), transformed with the vector p21b_ArEt, which is encoding bovine CYP21A2 as well as arh1 and etp1fd, by using resting cells in a volume of 500 mL and a cell density of 72 g L−1. Samples were taken after certain time points and analyzed via HPLC to examine the temporal premedrol increase

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