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Fig. 7 | Microbial Cell Factories

Fig. 7

From: A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol

Fig. 7

Determination of dissociation constants (KD values) with different substrates titrated to CYP21A2. Dissociation constants for a medrane, b progesterone and c 17OH-progesterone were determined by titration of bovine CYP21A2 with increasing concentrations of the particular substrate leading to a typical type I shift. Hyperbolic regression resulted in the respective KD values, indicating the extent of the binding affinity towards the enzyme

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