Fig. 5

Time-dependent premedrol formation by different CYP21A2 based whole-cell systems. Whole-cell biotransformation was performed with all constructed systems simultaneously by using resting cells. Samples were taken after 0, 2, 4, 10 and 24 h and analyzed via HPLC. The premedrol yield at several time points was determined for each system. All values represent the mean of triplicates with the respective standard deviation