Fig. 1

Protein analysis of purified bovine CYP21A2 after three chromatographic steps. a SDS-PAGE analysis and protein staining shows the purification steps of CYP21A2 via IMAC, DEAE- and SP-Sepharose resulting in a single protein band with a size of approx. 55 kDa (estimated weight: 54.6 kDa); b Difference spectroscopy of purified bovine CYP21A2 was performed showing a typical absorption maximum at 450 nm in the reduced state in complex with CO