Table 1 S. cerevisiae strains and plasmids used in this study
From: Optimization of yeast-based production of medicinal protoberberine alkaloids
Plasmid | Description | Source |
|---|---|---|
pCS6 | pRS314, CEN/ARS vector, TRP1 selectable marker | [50] |
pCS8 | pRS316, CEN/ARS vector, URA3 selectable marker | [50] |
pCS952 | 2 μ vector, P TEF1 -TfS9OMT-T CYC1 , URA3 selectable marker | [13] |
pCS953 | 2 μ vector, P TEF1 -TfS9OMT-T CYC1 , P TEF1 -TfCAS-T CYC1 , URA3 selectable marker | [13] |
pCS1018 | 2 μ vector, P TEF1 -PsBBE-T CYC1 , TRP1 selectable marker | [13] |
pCS3194 | 2 μ vector, P TDH3 -repaired TfCAS-EGFP-T CYC1 , TRP1 selectable marker | This work |
pCS3195 | CEN/ARS vector, P TDH3 -repaired TfCAS-EGFP-T CYC1 , TRP1 selectable marker | This work |
pCS3196 | CEN/ARS vector, P TDH3 -EGFP-T CYC1 , TRP1 selectable marker | This work |
pCS3197 | 2 μ vector, P TDH3 -EGFP-T CYC1 , TRP1 selectable marker | This work |
pCS3070 | CEN/ARS vector, P TDH3 -repaired TfCAS-T CYC1 , TRP1 selectable marker | This work |
pCS3071 | CEN/ARS vector, P TDH3 -TfCAS from pCS952-T CYC1 , TRP1 selectable marker | This work |
pCS3072 | 2 μ vector, P TDH3 -TfCAS from pCS952-T CYC1 , TRP1 selectable marker | This work |
pCS3409 | CEN/ARS vector, P TEF1 -repaired TfCAS-T CYC1 , TRP1 selectable marker | This work |
pCS3100 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -TfS9OMT-T CYC1 , P TDH3 -TfCAS-T ADH1 , TRP1 selectable marker | This work |
pCS3198 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yCjS9OMT-T CYC1 , P TDH3 -TfCAS-T ADH1 , TRP1 selectable marker | This work |
pCS3199 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -TfCAS-T ADH1 , TRP1 selectable marker | This work |
pCS3200 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yPsCAS-T ADH1 , TRP1 selectable marker | This work |
pCS3201 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yAmCAS-T ADH1 , TRP1 selectable marker | This work |
pCS3202 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yCjCAS-T ADH1 , TRP1 selectable marker | This work |
pCS3203 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yCjCAS-T ADH1 , P TPI1 -yBwSTOX-T STE2 , TRP1 selectable marker | This work |
pCS3204 | YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yCjCAS-T ADH1 , P HXT7 -yBwSTOX-T PGK1 , TRP1 selectable marker | This work |
Strain | Description | Source |
|---|---|---|
CSY3 | W303 (Matα, ade2–1 ura3–1 his3–11, 15 trp1–1 leu2–3, 112 can 1–100) | K. Weis |
CSY288 | CSY3 his3::P TEF1 -Ps6OMT-T CYC1 , leu2::P TEF1 -PsCNMT-T CYC1 , ura3::P TEF1 -Ps4′OMT-T CYC1 | [13] |
CSY410 | CSY288 trp1::P TEF1 -CPR-T CYC1 , pCS953, pCS1018 | [13] |
CSY835 | CSY288 trp1::P TEF1 -CPR-T CYC1 | This work |
CSY844 | CSY288 trp1::P TEF1 -CPR-T CYC1 , lys2::P TEF1 -PsBBE-T CYC1 | [14] |
CSY1005 | CSY3, pCS3194 | This work |
CSY1006 | CSY3, pCS3195 | This work |
CSY1007 | CSY3 fcy1::P TDH3 -TfCAS-EGFP-T CYC1 , pCS6 | This work |
CSY1008 | CSY3, pCS3196 | This work |
CSY1009 | CSY3, pCS3197 | This work |
CSY1010 | CSY3 fcy1::P TDH3 -EGFP-T CYC1 , pCS6 | This work |
CSY1011 | CSY844, pCS6, pCS953 | This work |
CSY1012 | CSY844, pCS952, pCS3072 | This work |
CSY1013 | CSY844, pCS952, pCS3071 | This work |
CSY1083 | CSY844, pCS952, pCS3409 | This work |
CSY1014 | CSY844, pCS952, pCS3070 | This work |
CSY1016 | CSY835, pCS8, pCS3100 | This work |
CSY1017 | CSY844, pCS8, pCS3100 | This work |
CSY1018 | CSY844, pCS8, pCS3198 | This work |
CSY1019 | CSY844, pCS8, pCS3199 | This work |
CSY1020 | CSY844, pCS952, pCS3199 | This work |
CSY1021 | CSY844, pCS952, pCS3200 | This work |
CSY1022 | CSY844, pCS952, pCS3201 | This work |
CSY1023 | CSY844, pCS952, pCS3202 | This work |
CSY1024 | CSY844 XI-3::P TEF1 -yCjCAS-T CYC1 , pCS952, pCS3202 | XI-3 locus from [51] This work |
CSY1025 | CSY844, pCS952, pCS3203 | This work |
CSY1026 | CSY844, pCS952, pCS3204 | This work |