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Table 1 S. cerevisiae strains and plasmids used in this study

From: Optimization of yeast-based production of medicinal protoberberine alkaloids

Plasmid Description Source
pCS6 pRS314, CEN/ARS vector, TRP1 selectable marker [50]
pCS8 pRS316, CEN/ARS vector, URA3 selectable marker [50]
pCS952 2 μ vector, P TEF1 -TfS9OMT-T CYC1 , URA3 selectable marker [13]
pCS953 2 μ vector, P TEF1 -TfS9OMT-T CYC1 , P TEF1 -TfCAS-T CYC1 , URA3 selectable marker [13]
pCS1018 2 μ vector, P TEF1 -PsBBE-T CYC1 , TRP1 selectable marker [13]
pCS3194 2 μ vector, P TDH3 -repaired TfCAS-EGFP-T CYC1 , TRP1 selectable marker This work
pCS3195 CEN/ARS vector, P TDH3 -repaired TfCAS-EGFP-T CYC1 , TRP1 selectable marker This work
pCS3196 CEN/ARS vector, P TDH3 -EGFP-T CYC1 , TRP1 selectable marker This work
pCS3197 2 μ vector, P TDH3 -EGFP-T CYC1 , TRP1 selectable marker This work
pCS3070 CEN/ARS vector, P TDH3 -repaired TfCAS-T CYC1 , TRP1 selectable marker This work
pCS3071 CEN/ARS vector, P TDH3 -TfCAS from pCS952-T CYC1 , TRP1 selectable marker This work
pCS3072 2 μ vector, P TDH3 -TfCAS from pCS952-T CYC1 , TRP1 selectable marker This work
pCS3409 CEN/ARS vector, P TEF1 -repaired TfCAS-T CYC1 , TRP1 selectable marker This work
pCS3100 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -TfS9OMT-T CYC1 , P TDH3 -TfCAS-T ADH1 , TRP1 selectable marker This work
pCS3198 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yCjS9OMT-T CYC1 , P TDH3 -TfCAS-T ADH1 , TRP1 selectable marker This work
pCS3199 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -TfCAS-T ADH1 , TRP1 selectable marker This work
pCS3200 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yPsCAS-T ADH1 , TRP1 selectable marker This work
pCS3201 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yAmCAS-T ADH1 , TRP1 selectable marker This work
pCS3202 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yCjCAS-T ADH1 , TRP1 selectable marker This work
pCS3203 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yCjCAS-T ADH1 , P TPI1 -yBwSTOX-T STE2 , TRP1 selectable marker This work
pCS3204 YAC vector pYES1L, P PGK1 -PsBBE-T PHO5 , P TEF1 -yPsS9OMT-T CYC1 , P TDH3 -yCjCAS-T ADH1 , P HXT7 -yBwSTOX-T PGK1 , TRP1 selectable marker This work
Strain Description Source
CSY3 W303 (Matα, ade21 ura31 his311, 15 trp11 leu23, 112 can 1100) K. Weis
CSY288 CSY3 his3::P TEF1 -Ps6OMT-T CYC1 , leu2::P TEF1 -PsCNMT-T CYC1 , ura3::P TEF1 -Ps4′OMT-T CYC1 [13]
CSY410 CSY288 trp1::P TEF1 -CPR-T CYC1 , pCS953, pCS1018 [13]
CSY835 CSY288 trp1::P TEF1 -CPR-T CYC1 This work
CSY844 CSY288 trp1::P TEF1 -CPR-T CYC1 , lys2::P TEF1 -PsBBE-T CYC1 [14]
CSY1005 CSY3, pCS3194 This work
CSY1006 CSY3, pCS3195 This work
CSY1007 CSY3 fcy1::P TDH3 -TfCAS-EGFP-T CYC1 , pCS6 This work
CSY1008 CSY3, pCS3196 This work
CSY1009 CSY3, pCS3197 This work
CSY1010 CSY3 fcy1::P TDH3 -EGFP-T CYC1 , pCS6 This work
CSY1011 CSY844, pCS6, pCS953 This work
CSY1012 CSY844, pCS952, pCS3072 This work
CSY1013 CSY844, pCS952, pCS3071 This work
CSY1083 CSY844, pCS952, pCS3409 This work
CSY1014 CSY844, pCS952, pCS3070 This work
CSY1016 CSY835, pCS8, pCS3100 This work
CSY1017 CSY844, pCS8, pCS3100 This work
CSY1018 CSY844, pCS8, pCS3198 This work
CSY1019 CSY844, pCS8, pCS3199 This work
CSY1020 CSY844, pCS952, pCS3199 This work
CSY1021 CSY844, pCS952, pCS3200 This work
CSY1022 CSY844, pCS952, pCS3201 This work
CSY1023 CSY844, pCS952, pCS3202 This work
CSY1024 CSY844 XI-3::P TEF1 -yCjCAS-T CYC1 , pCS952, pCS3202 XI-3 locus from [51]
This work
CSY1025 CSY844, pCS952, pCS3203 This work
CSY1026 CSY844, pCS952, pCS3204 This work