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Table 3 Biomass, GSH concentration, GSH content and molar ratio of γ-GC to GSH of various strains in flask cultures with or without addition of amino acid precursors (AA)

From: Three-pathway combination for glutathione biosynthesis in Saccharomyces cerevisiae

Strains

Biomass (DCW)a

GSH concentration (mg/L)

GSH content (%)

Molar ratio of γ-GC to GSH (%)

W303-1b

9.14 ± 0.48

98.75 ± 7.30

1.09 ± 0.14

6.74 ± 0.24

W303-1b/F

9.09 ± 0.13

186.77 ± 3.19

2.05 ± 0.06

10.29 ± 0.87

W303-1b/G

9.56 ± 0.26

145.72 ± 14.87

1.52 ± 0.14

9.72 ± 0.93

W303-1b/P

9.23 ± 0.32

111.82 ± 11.34

1.21 ± 0.09

8.58 ± 2.53

W303-1b/P′

8.74 ± 0.44

104.24 ± 9.86

1.19 ± 0.08

7.34 ± 0.42

W303-1b/FG

9.35 ± 0.38

206.70 ± 7.23

2.21 ± 0.02

11.26 ± 0.67

W303-1b/FP

9.04 ± 0.20

190.57 ± 1.56

2.11 ± 0.05

10.92 ± 0.93

W303-1b/FGP

9.26 ± 0.07

216.50 ± 11.46

2.34 ± 0.13

9.97 ± 0.40

W303-1b/F (AA)

8.58 ± 0.09

252.33 ± 28.62

2.94 ± 0.36

9.65 ± 0.51

W303-1b/FGP (AA)

8.38 ± 0.37

316.77 ± 45.69

3.77 ± 0.43

9.50 ± 1.43

  1. aDCW is calculated from OD600 values (OD600 = 10 corresponds to 1.19 g/L DCW). The values given are mean values ± standard deviation