Table 2 Strains and plasmids used in this study
From: Three-pathway combination for glutathione biosynthesis in Saccharomyces cerevisiae
Strains or plasmids | Relevant properties | Source or references |
|---|---|---|
Strains | ||
E. coli Trans1-T1 | F− φ80(lacZ) ΔM15 ΔlacX74 hsdR(rK− mK+) ΔrecA1398 endA1 tonA | Our lab |
S. cerevisiae W303-1b | MATα ade2-1 leu2-3,112 his3-11,15 ura3-1 trp1-1 | Our lab |
W303-1b/Δgsh1 | W303-1b derivative with pΔgsh1h, GSH1::HygB | This study |
W303-1b/Δgsh1/pro1 | W303-1b derivative with pΔgsh1h-pro1, GSH1::P gap -PRO1-T pgk1 | This study |
W303-1b/Δgsh1/proB | W303-1b derivative with pΔgsh1h-proB, GSH1::P gap -proB-T pgk1 | This study |
W303-1b/F/GAP (W303-1b/F)a | W303-1b derivative with pδGAPg-gshF | This study |
W303-1b/F/GAP′ | W303-1b derivative with pδGAP′g-gshF | This study |
W303-1b/F/PGK1 | W303-1b derivative with pδPGK1g-gshF | This study |
W303-1b/F/GAL1 | W303-1b derivative with pδGAL1g-gshF | This study |
W303-1b/Δgsh1/F | W303-1b/F derivative with pΔgsh1h, GSH1::HygB | This study |
W303-1b/G | W303-1b derivative with pδGAPh-gsh2gsh1 | This study |
W303-1b/P | W303-1b derivative with pδGAPg-pro1gshB | This study |
W303-1b/P′ | W303-1b derivative with pδGAPg-proBgshB | This study |
W303-1b/FF | W303-1b/F derivative with pδGAPg-gshF | This study |
W303-1b/FG | W303-1b/F derivative with pδGAPh-gsh2gsh1 | This study |
W303-1b/FP | W303-1b/F derivative with pδGAPg-pro1gshB | This study |
W303-1b/FGP | W303-1b/FG derivative with pδGAPg-pro1gshB | This study |
Plasmids | ||
pG | pBluescript II KS(+) derivative with homologous region of GSH1 | Our lab |
pΔgsh1h | pBluescript II KS(+) derivative with homologous region of GSH1, HygBr | This study |
pΔgsh1h-pro1 | pG derivative with P gap , PRO1, T pgk1 , HygBr | This study |
pΔgsh1h-proB | pG derivative with P gap , proB, T pgk1 , HygBr | This study |
pδGAPg | pBluescript II KS(+) derivative with homologous δ region, P gap , T pgk1 , G418r | Our lab |
pδGAP′g | pBluescript II KS(+) derivative with homologous δ region, P gap′, T pgk1 , G418r | Our lab |
pδPGK1g | pBluescript II KS(+) derivative with homologous δ region, P pgk1 , T pgk1 , G418r | Our lab |
pδGAL1g | pBluescript II KS(+) derivative with homologous δ region, P gal1 , T pgk1 , G418r | Our lab |
pδGAPh | pBluescript II KS(+) derivative with homologous δ region, P gap , T pgk1 , HygBr | Our lab |
pδGAPg-gshF | pδGAPg derivative with A. pleuropneumoniae gshF, G418r | This study |
pδGAP′g-gshF | pδGAP′g derivative with A. pleuropneumoniae gshF, G418r | This study |
pδPGK1g-gshF | pδPGK1 g derivative with A. pleuropneumoniae gshF, G418r | This study |
pδGAL1g-gshF | pδGAL1 g derivative with A. pleuropneumoniae gshF, G418r | This study |
pδGAPh-gsh2gsh1 | pδGAPh derivative with GSH2-GSH1 from S. cerevisiae, HygBr | This study |
pδGAPg-pro1gshB | pδGAPg derivative with PRO1-gshB from S. cerevisiae and E. coli, respectively, G418r | This study |
pδGAPg-proBgshB | pδGAPg derivative with proB-gshB from E. coli, G418r | This study |