Table 1 Bacterial strains and plasmids used in this study
Strain/plasmid | Relevant characteristics | Source/reference |
|---|---|---|
Plasmid | ||
 pSBAC | aacIII(IV), oriT, attP-int, backbone of pCC1BAC | [12] |
 pSATNI | Modified pSBAC which deleted attP-int and inserted Kan R and tmcI fragment | This work |
 pTMC | pSATNI with 85 kb DNA insert containing whole tmc gene cluster | This work |
 pMMBL101 | pTMC with attP-int | This work |
 pMMBL102 | pMMBL101 which replaced Apr R into Spe R | This work |
E. coli | ||
 EPI300 | F- mcrA-D(mrr-hsdRMS-mcrBC) trfA host for cloning and amplification of various BAC vectors and constructs derived from it | Epicenter |
 S17-1 | E. coli host for transferring various plasmids into Streptomyces via conjugation |  |
 ET12567/pUZ8002 | E. coli host for transferring various plasmids into Streptomyces via conjugation |  |
Streptomyces sp. | ||
 CK4412 | Original TMC-producing strain | [17] |
 CK4412-2XB | CK4412 with pSATNI and XbaI recognition sequences in both flanking region of TMC biosynthetic gene cluster | This work |
 TMC001 | pMMBL101-containing CK4412 | This work |
Streptomyces lividans | ||
 TK21 | Non TMC-producing strain |  |
 TMC002 | TK21 with pMMBL101 | This work |
Streptomyces coelicolor | ||
 M145 | Non TMC-producing strain |  |
 TMC003 | M145 with pMMBL101 | This work |
 TMC004 | pMMBL102-containing S. coelicolor TMC003 | This work |