Fig. 6From: Precise cloning and tandem integration of large polyketide biosynthetic gene cluster using Streptomyces artificial chromosome systemTranscripts analysis of tmc-overexpressed CK4412 (CK4412-TMC001). The RNA sample was taken day 5. Three biosynthetic genes (tmcB, mcC, and tmcJ) and two pathway specific regulatory genes (tmcN and tmcT) were amplified. Each gene was amplified in duplicate. Error rates were calculated by standard deviation. White square CK4412 WT; black square CK4412-TMC001Back to article page