Fig. 2

Construction of plasmids for expression. The P43 promoter from B. subtilis 168 chromosomal DNA was introduced into the shuttle vector pUBC19 between the BamHI and PstI sites, and the recombinant plasmid was defined as pUBC19-P43. Genes for expression were PCR-amplified and were spliced by overlap extension. A KpnI site was introduced into the forward primer of patB, and a PstI site was introduced into the reverse primer of dhaS. The spliced fragment was ligated to the pUBC19-P43 plasmid in the KpnI and PstI sites, and the recombinant plasmid was named pUBC19-P43-E