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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial Bacillus amyloliquefaciens SQR9

Fig. 1

a Transcriptional levels of genes up regulated in the SQR9 grown with tryptophan relative to non-tryptophan treatment, as evaluated by qPCR. B. amyloliquefaciens SQR9 was grown in Landy medium with or without tryptophan for 65 h. The recA gene of SQR9 was used as an internal reference gene. Bars represent standard deviations of three biological replicates. b IAA production of the wild-type SQR9 and its derived strains. Strains were grown in Landy medium supplied with 3 mM tryptophan for 72 h. The bacterial strains ΔysnE-c, ΔdhaS-c, ΔyclC-c and ΔyhcX-c are complementary strains for the corresponding mutants. Different letters indicate significant differences (P < 0.05). c Quantification of the IAA produced by over-expression strains. Strains were cultured in Landy medium supplied with 3 mM tryptophan for IAA production. Bacteria strains are as follows: SQR9-E, pUBC19-P43-E in B. amyloliquefaciens SQR9; SQR9-CK, pUBC19-P43 in B. amyloliquefaciens SQR9; 168-E, pUBC19-P43-E in B. subtilis 168; and 168-CK, pUBC19-P43 in B. subtilis 168. Different letters indicate significant differences (P < 0.05). d Quantification of the IAA produced by sole gene expression strains. Strains were cultured in Landy medium supplied with 3 mM tryptophan for IAA production. Bacteria strains are as follows: patB-E, pUBC19-P43-patB in B. amyloliquefaciens SQR9; yclC-E, pUBC19-P43-yclC in B. amyloliquefaciens SQR9; dhaS-E, pUBC19-P43-dhaS in B. amyloliquefaciens SQR9; and SQR9-CK, pUBC19-P43 in B. amyloliquefaciens SQR9. Different letters indicate significant differences (P < 0.05)

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