Fig. 3

Surface–activated refolding of recombinant antibodies. Bacteria are first forced to produce inclusion bodies formed by recombinant antibodies fused to peptides affine for plastic. Once denatured, each single polypeptide adhere separately onto plastic plate surface by means of the peptide tag. The denatured polypeptides efficiently refold (solid-phase refolding) into functional antibodies because the unproductive interactions involving hydrophobic patches present at the surface of different folding intermediates are prevented