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Table 3 The plasmids and E. coli strains utilized in this study

From: Engineering E. coliE. coli cocultures for production of muconic acid from glycerol

Plasmid

Description

Source

pET21c

T7 promoter, AmpR

Novagen

pET28a

T7 promoter, KanR

Novagen

pHACM-rpoA14

A gTME plasmid carrying a mutated alpha subunit of RNA polymerase for enhancing the shikimate pathway

[30]

pAO

pET21c carrying the catA and auxiliary ACIAD1443 genes from Acinetobacter calcoaceticus

[20]

pAOM

pACYCDuet-1 carrying the catA gene and ACIAD1443 genes

[20]

pY

pET28a carrying the aroY gene from Klebsiella pneumoniae

[20]

pZ

pET28a carrying the aroZ gene from Klebsiella pneumoniae

[20]

pYZS

pET28a carrying the aroY and aroZ genes from Klebsiella pneumonia and the E. coli shiA gene

[20]

pYfsZ

pET28a carrying the aroY and fused shiA-aroZ genes

This study

pYpZ

pY carrying aroZ that has a phage gIII leading sequence to localize AroZ enzyme into periplasm

This study

pG-KJE

A plasmid carrying E. coli chaperone dnaK-dnaJ-grpE and groES-groEL genes, CmR (only groES-groEL genes were induced for expression)

Clontech

pII

A low copy number plasmid expressing weak green fluorecence protein, KanR

[31]

pCS

pACYCDuet-1 carrying the shiA gene that is under the control of a constitutive lacuv5 promoter

[20]

pCS2

pCDFDuet-1 carrying the shiA gene that is under the control of a constitutive lacuv5 promoter

[20]

pCYZAO

pUC57(Kan) carrying the aroY, aroZ, catA and ACIAD1443 genes that are under the control of a constitutive Zymomonas mobilis pyruvate decarboxylase promoter

[20]

Strain

Description

Source

P5

E. coli K12 ΔpheA ΔtyrR lacZ::PLtetO-1-tyrA fbr aroG fbr tyrR::PLtetO-1-tyrA fbr aroG fbr hisH(L82R) ΔaroE ΔydiB

[20]

P5g

P5 harboring pHACM-rpoA14, pAO and pYZ

[20]

P5s

P5 harboring pHACM-rpoA14, pAO and pYZS

[20]

P5fs

P5 harboring pHACM-rpoA14, pAO and pYfsZ

This study

P5pZ

P5 harboring pHACM-rpoA14, pAO and pYpZ

This study

P5cS

P5 harboring pHACM-rpoA14, pCYZAO and pCS2

This study

P5.2

P5 harboring pII and pHACM-rpoA14

This study

BLS

E. coli BL21(DE3) harboring pHACM-rpoA14, pAO and pYZS

This study

BLH

E. coli BL21(DE3) harboring pG-KJE, pAO and pYZS

This study

BS

E. coli BL21(DE3) harboring pYZS and pAOM

This study

BC

E. coli BL21(DE3) harboring pCYZAO and pCS

This study