From: Engineering E. coli–E. coli cocultures for production of muconic acid from glycerol
Plasmid | Description | Source |
---|---|---|
pET21c | T7 promoter, AmpR | Novagen |
pET28a | T7 promoter, KanR | Novagen |
pHACM-rpoA14 | A gTME plasmid carrying a mutated alpha subunit of RNA polymerase for enhancing the shikimate pathway | [30] |
pAO | pET21c carrying the catA and auxiliary ACIAD1443 genes from Acinetobacter calcoaceticus | [20] |
pAOM | pACYCDuet-1 carrying the catA gene and ACIAD1443 genes | [20] |
pY | pET28a carrying the aroY gene from Klebsiella pneumoniae | [20] |
pZ | pET28a carrying the aroZ gene from Klebsiella pneumoniae | [20] |
pYZS | pET28a carrying the aroY and aroZ genes from Klebsiella pneumonia and the E. coli shiA gene | [20] |
pYfsZ | pET28a carrying the aroY and fused shiA-aroZ genes | This study |
pYpZ | pY carrying aroZ that has a phage gIII leading sequence to localize AroZ enzyme into periplasm | This study |
pG-KJE | A plasmid carrying E. coli chaperone dnaK-dnaJ-grpE and groES-groEL genes, CmR (only groES-groEL genes were induced for expression) | Clontech |
pII | A low copy number plasmid expressing weak green fluorecence protein, KanR | [31] |
pCS | pACYCDuet-1 carrying the shiA gene that is under the control of a constitutive lacuv5 promoter | [20] |
pCS2 | pCDFDuet-1 carrying the shiA gene that is under the control of a constitutive lacuv5 promoter | [20] |
pCYZAO | pUC57(Kan) carrying the aroY, aroZ, catA and ACIAD1443 genes that are under the control of a constitutive Zymomonas mobilis pyruvate decarboxylase promoter | [20] |
Strain | Description | Source |
---|---|---|
P5 | E. coli K12 ΔpheA ΔtyrR lacZ::PLtetO-1-tyrA fbr aroG fbr tyrR::PLtetO-1-tyrA fbr aroG fbr hisH(L82R) ΔaroE ΔydiB | [20] |
P5g | P5 harboring pHACM-rpoA14, pAO and pYZ | [20] |
P5s | P5 harboring pHACM-rpoA14, pAO and pYZS | [20] |
P5fs | P5 harboring pHACM-rpoA14, pAO and pYfsZ | This study |
P5pZ | P5 harboring pHACM-rpoA14, pAO and pYpZ | This study |
P5cS | P5 harboring pHACM-rpoA14, pCYZAO and pCS2 | This study |
P5.2 | P5 harboring pII and pHACM-rpoA14 | This study |
BLS | E. coli BL21(DE3) harboring pHACM-rpoA14, pAO and pYZS | This study |
BLH | E. coli BL21(DE3) harboring pG-KJE, pAO and pYZS | This study |
BS | E. coli BL21(DE3) harboring pYZS and pAOM | This study |
BC | E. coli BL21(DE3) harboring pCYZAO and pCS | This study |