Bacterial strain, plasmid or primer names | Relevant characteristic or sequence | Source or enzyme site |
---|---|---|
Strains | ||
 B. amyloliquefaciens | ||
  B10-127 | Host strain | Lab stock |
  DH | B10-127 with pMA5 -dhaD | This study |
  GL | B10-127 with pMA5-gldA | This study |
  GA | B10-127 with pMA5-acr-HapII-dhaD | This study |
  GAR | B10-127 with pMA5-acr-HapII-dhaD-PbdhA-alsR | This study |
  K. pneumoniae ATCC 25955 | Source of dhaD gene | Lab stock |
  E. coli JM109 |  | Lab stock |
 Plasmids | ||
  pMA5-HapII | Expression vector (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | Lab stock |
  pMA5-acr | pMA5-HapII with acr (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | Lab stock |
  pMA5-PbahA-alsR | pMA5 containing PbahA-alsR (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | Lab stock |
  pMA5-HpaII-alsR | pMA5 containing HpaII-alsR (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | Lab stock |
  pMA5-gldA | pMA5-HapII with gldA (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
  pMA5-dhaD | pMA5-HapII with dhaD (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
  pMA5-acr-HapII-dhaD | Apr, Kanr; pMA5-HapII with acr and HapII-dhaD (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
  pMA5-acr-HapII-dhaD-PbahA-alsR | Apr, Kanr; pMA5-acr-HapII-dhaD with PbahA-alsR (in E. coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
 Primers | ||
  P1 | 5′-CGGGATCCATGAAGCCTGAAGATATCG-3′ | BamHI |
  P2 | 5′-CGACGCGTCTATCCTGTCTTTTGCGC-3′ | MluI |
  P3 | 5′-CGACGCGTTTTTGAGTGATCTTCTC-3′ | MluI |
  P4 | 5′-CGGGATCCATGGATCGCATTATTCAATC-3′ | BamHI |
  P5 | 5′-CGACGCGTTTATTCCCATTCCTGCAGG-3′ | MluI |