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Table 3 Strains, plasmids and primers used in this study

From: Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens

Bacterial strain, plasmid or primer names

Relevant characteristic or sequence

Source or enzyme site

Strains

 B. amyloliquefaciens

  B10-127

Host strain

Lab stock

  DH

B10-127 with pMA5 -dhaD

This study

  GL

B10-127 with pMA5-gldA

This study

  GA

B10-127 with pMA5-acr-HapII-dhaD

This study

  GAR

B10-127 with pMA5-acr-HapII-dhaD-PbdhA-alsR

This study

  K. pneumoniae ATCC 25955

Source of dhaD gene

Lab stock

  E. coli JM109

 

Lab stock

 Plasmids

  pMA5-HapII

Expression vector (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

Lab stock

  pMA5-acr

pMA5-HapII with acr (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

Lab stock

  pMA5-PbahA-alsR

pMA5 containing PbahA-alsR (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

Lab stock

  pMA5-HpaII-alsR

pMA5 containing HpaII-alsR (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

Lab stock

  pMA5-gldA

pMA5-HapII with gldA (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

This study

  pMA5-dhaD

pMA5-HapII with dhaD (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

This study

  pMA5-acr-HapII-dhaD

Apr, Kanr; pMA5-HapII with acr and HapII-dhaD (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

This study

  pMA5-acr-HapII-dhaD-PbahA-alsR

Apr, Kanr; pMA5-acr-HapII-dhaD with PbahA-alsR (in E. coli, Apr; in B. amyloliquefaciens, Kanr)

This study

 Primers

  P1

5′-CGGGATCCATGAAGCCTGAAGATATCG-3′

BamHI

  P2

5′-CGACGCGTCTATCCTGTCTTTTGCGC-3′

MluI

  P3

5′-CGACGCGTTTTTGAGTGATCTTCTC-3′

MluI

  P4

5′-CGGGATCCATGGATCGCATTATTCAATC-3′

BamHI

  P5

5′-CGACGCGTTTATTCCCATTCCTGCAGG-3′

MluI

  1. Underlined nucleotides are the restriction enzyme sites