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Table 1 Primers for the target proteins cloning

From: BrkAutoDisplay: functional display of multiple exogenous proteins on the surface of Escherichia coli by using BrkA autotransporter

Primer name

Sequence

Target protein to be displayed

Restriction sites

F1

5′-CGGGATCCATGGTGAGCAAGGGCGAG-3′

GFP

BamHI and XhoI

R1

5′-CCGCTCGAGCCCTTGTACAGCTCGTCCA-3′

F2

5′-CGGGATCCATGACACTTGAAAAATTTGTGG-′

CotA

BamHI and XhoI

R2

5′-CCGCTCGAGCCTTTATGGGGATCAGTTA-3′

F3

5′-CGGGATCCATGGGCAAAGTTCCAGAAGAAG-3′

ECH-9

BamHI and KpnI

R3

5′-GGGGTACCTAGTTTTGATGACATCAGTTTG-3′

F4

5′-CGGGATCCCGGCCCTGGCAGATCCTGA-3′

AtaPT

BamHI and KpnI

R4

5′-GGGGTACCCACACGTGCGACATTTCCCGCAA-3′

F5

5′-CGGGATCCATGTCTGGAAAAGTGGTTAG-3′

ECH

BamHI and KpnI

R5

5′-GGGGTACCTTTCTTGACGACCGCTTCGAG-3′

F6

5′-CGGAATTCATAAATACCACCCA AAAGATTATTC-3′

EstPc

EcoRI and KpnI

R6

5′-GGGGTACCGTTCTTTAACCCTTCACGAAACGC-3′

F7

5′-CGGAATTCATGATCAAAC AGACGTTGTTTGTACC-3′

PalA

EcoRI and KpnI

R7

5′-GGGGTACCTTCGTCCTGA TGAGCGCGCA ACGT-3′

F8

5′-CGGAATTCATGGATATCGGAATTAATTCGGATCC-3′

ATscFv

EcoRI and XhoI

R8

5′-CCGCTCGAGCCCCGTTTTATTTCCAACTTTG-3′