Table 2 Kinetic parameters of WT and mutant MPH
From: Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy
Enzyme | k cat (min−1) | K m (μM) | k cat/K m (μM−1 min−1) |
|---|---|---|---|
CHBD-MPH | 215.20 ± 7.30 | 86.85 ± 5.85 | 2.48 |
CHBD-QR | 329.50 ± 12.92 | 119.10 ± 8.26 | 2.77 |
CHBD-DQR | 572.80 ± 12.30 | 168.50 ± 7.57 | 3.40 |