Fig. 3
From: Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy
Enzyme activity and growth kenetics of the recombinant P. pastoris clones in shake-flask culture. Transformants expressing MPH (solid circle), CHBD-MPH (hollow circle), CHBD-QR (solid triangle), or CHBD-DQR (hollow triangle) were induced by methanol for the indicated times on the x-axis, and MPH activity was determined using standard enzyme assays (as indicated as y-axis). a Extracellular MPH activity of the recombinant P. pastoris clones; b intracellular MPH activity of the recombinant P. pastoris clones; c Growth kenetics of the recombinant P. pastoris clones. Enzyme activity is expressed as the mean of three samples, and error bars indicate standard deviations (SD)