Fig. 1From: Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy Box–whisker plot of MPH activity of single factors mutants in culture supernatants of P. pastoris. MPH activity of transformants in culture supernatants was determined using standard enzyme assays after 48 h of induction by methanol. a wild-type MPH and mutants of N-terminal tag-fusion MPHs: CHBD-MPH, GST-MPH, MBP-MPH, and CBD-MPH; b wild-type MPH and KKXX motif mutants: K284R, K285R, and K284R/285R; c wild-type MPH and acidic stability enhanced mutant: K277D. Boxes denote the interquartile range (IQR) between the first and third quartiles, while the line within the box denotes the median. Whiskers denote the lowest and highest values within 1.5-fold of the IQR from the first and third quartiles while circles denote outliersBack to article page