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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy

Fig. 1

Box–whisker plot of MPH activity of single factors mutants in culture supernatants of P. pastoris. MPH activity of transformants in culture supernatants was determined using standard enzyme assays after 48 h of induction by methanol. a wild-type MPH and mutants of N-terminal tag-fusion MPHs: CHBD-MPH, GST-MPH, MBP-MPH, and CBD-MPH; b wild-type MPH and KKXX motif mutants: K284R, K285R, and K284R/285R; c wild-type MPH and acidic stability enhanced mutant: K277D. Boxes denote the interquartile range (IQR) between the first and third quartiles, while the line within the box denotes the median. Whiskers denote the lowest and highest values within 1.5-fold of the IQR from the first and third quartiles while circles denote outliers

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