Optimization of ethylene glycol production from (d)-xylose via a synthetic pathway implemented in Escherichia coli

Table 6 Plasmids used in this study

Name	Relevant characteristics	References
pGEM-T	Amp^R, used for PCR fragment subcloning	Promega
pACT3	Cm^R	[33]
pEXT20	Amp^R	[33]
pCP20	Amp^R, plasmid used for removing Kan cassette	[34]
pEXT20-khkC-aldoB	pEXT20 derivative carrying both H. sapiens khk-c gene from Asipu et al., 2003 and aldoB gene	[8]
pACT3-gldA	pACT3 derivative carrying gldA gene from E. coli	This work
pACT3-yqhD	pACT3 derivative carrying yqhD gene from E. coli	This work
pACT3-fucO	pACT3 derivative carrying fucO gene from E. coli	This work

ISSN: 1475-2859