Table 5 Primers used in this study
Primer | Sequence |
|---|---|
Cloning of gldA | |
gldA_rbs_f | CCTCTAGAGTCGACCTGCAGAGGAGGATTCATATGGACCGCATTA |
gldA_rbs_r | GCCAAAACAGAAGCTTTTATTCCCACTCTTGCAGG |
Cloning of fucO | |
fucO_rbs_f | TTGGATCCAGGAGGATTCATATGGCTAACAGAATGATTCT |
fucO_rbs_r | TTAAGCTTTTACCAGGCGGTATGGTAA |
Cloning of yqhD | |
yqhD_rbs_f | CCTCTAGAGTCGACCTGCAGAGGAGGATTCATATGAACAACTTTA |
yqhD_rbs_r | GCCAAAACAGAAGCTTTTAGCGGGCGGCTTCGT |
Verification primers for gene knock-outs | |
xylB_loc_f | GTTATCGGTAGCGATACCGGGCATTTT |
xylB_loc_r | GGATCCTGAATTATCCCCCACCCGGTCAGGCA |
yqhD_loc_f | CGCCATACAACAAACGCACA |
yqhD_loc_r | CCAGATGCCAGCGGATAACA |
gldA_loc_f | CGGTTCAGGAGCTGCAAACGCTG |
gldA_loc_r | TAAGAGTCACAGATTCGACCTTC |
fucO_loc_f | ACAACATCATGGGCTTATCG |
KANseq_rev | ATGCGATGTTTCGCTTGGTG |
aldA_loc_f | TCATCCATGCATGGCAAACG |
aldA_loc_r | ACTGCCGAAGAGGTGAATAA |