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Table 3 The impact of variations in oxygen supply on the production of ethylene glycol

From: Optimization of ethylene glycol production from (d)-xylose via a synthetic pathway implemented in Escherichia coli

Strain name

Genotype and plasmids

Culture condition

YBiomass (g/g)

YEG (mol/mol)

YAcetate (mol/mol)

YFormate (mol/mol)

YSuccinate (mol/mol)

Pen325

ΔxylB ΔaldA

pEXT20-khkC-aldoB

1

0.12 ± 0.01

0.88 ± 0.01

0.00 ± 0.00

0.02 ± 0.02

0.00 ± 0.00

2

0.09 ± 0.00

0.87 ± 0.00

0.05 ± 0.01

0.13 ± 0.00

0.00 ± 0.00

3

0.06 ± 0.01

0.65 ± 0.00

0.70 ± 0.18

0.00 ± 0.00

0.00 ± 0.00

Pen332

ΔxylB ΔaldA

pEXT20-khkC-aldoB

pACT3-gldA

1

0.08 ± 0.01

0.52 ± 0.02

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

2

0.08 ± 0.02

0.74 ± 0.05

0.01 ± 0.00

0.04 ± 0.00

0.00 ± 0.00

3

0.04 ± 0.01

0.46 ± 0.03

0.30 ± 0.12

0.01 ± 0.00

0.19 ± 0.02

Pen333

ΔxylB ΔaldA

pEXT20-khkC-aldoB

pACT3-yqhD

1

0.11 ± 0.01

0.90 ± 0.01

0.00 ± 0.01

0.01 ± 0.00

0.00 ± 0.00

2

0.08 ± 0.00

0.79 ± 0.04

0.10 ± 0.14

0.11 ± 0.03

0.00 ± 0.00

3

0.03 ± 0.01

0.59 ± 0.10

0.50 ± 0.12

0.01 ± 0.00

0.17 ± 0.02

Pen641

ΔxylB ΔaldA

pEXT20-khkC-aldoB

pACT3-fucO

1

0.16 ± 0.01

0.94 ± 0.00

0.00 ± 0.00

0.07 ± 0.01

0.00 ± 0.00

2

0.08 ± 0.01

0.80 ± 0.00

0.18 ± 0.06

0.17 ± 0.08

0.00 ± 0.00

3

0.05 ± 0.00

0.61 ± 0.12

0.42 ± 0.11

0.01 ± 0.00

0.20 ± 0.07

  1. (1) 50 mL medium in 250 mL shake flasks, 200 rpm. (2) 100 mL medium in 250 mL shake flasks, 100 rpm. (3) anaerobic cultures, 200 rpm. Data is presented as means ± standard deviations of at least two independent experiments.