Table 2 Product yields (Y) of engineered E. coli strains expressing the synthetic pathway
Strain name | Deletions | Plasmids | YBiomass (g/g) | YEG (mol/mol) | YGA (mol/mol) | YAcetate (mol/mol) | YFormate (mol/mol) |
|---|---|---|---|---|---|---|---|
Pen877 | pEXT20-khkC-aldoB | 0.11 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 1.07 ± 0.02 | 0.20 ± 0.01 | |
Pen205 | ΔxylB | pEXT20-khkC-aldoB | 0.14 ± 0.00 | 0.45 ± 0.01 | 0.09 ± 0.00 | 0.00 ± 0.00 | 0.14 ± 0.01 |
Pen885 | ΔxylB | pEXT20-khkC-aldoB + pACT3-empty | 0.14 ± 0.00 | 0.33 ± 0.01 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.17 ± 0.01 |
Pen222 | ΔxylB | pEXT20-khkC-aldoB + pACT3-gldA | 0.15 ± 0.01 | 0.37 ± 0.00 | 0.00 ± 0.00 | 0.01 ± 0.00 | 0.13 ± 0.03 |
Pen223 | ΔxylB | pEXT20-khkC-aldoB + pACT3-yqhD | 0.18 ± 0.03 | 0.33 ± 0.00 | 0.03 ± 0.03 | 0.00 ± 0.00 | 0.22 ± 0.00 |
Pen644 | ΔxylB | pEXT20-khkC-aldoB + pACT3-fucO | 0.14 ± 0.01 | 0.36 ± 0.01 | 0.06 ± 0.00 | 0.01 ± 0.00 | 0.18 ± 0.00 |
Pen334 | ΔxylB ΔyqhD | pEXT20-khkC-aldoB | 0.14 ± 0.00 | 0.07 ± 0.01 | 0.04 ± 0.00 | 0.02 ± 0.00 | 0.01 ± 0.00 |
Pen325 | ΔxylB ΔaldA | pEXT20-khkC-aldoB | 0.12 ± 0.01 | 0.88 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.02 ± 0.02 |
Pen361 | ΔxylB ΔaldA ΔglcD | pEXT20-khkC-aldoB | 0.10 ± 0.00 | 0.87 ± 0.00 | 0.02 ± 0.00 | 0.00 ± 0.00 | 0.02 ± 0.00 |
Pen332 | ΔxylB ΔaldA | pEXT20-khkC-aldoB + pACT3-gldA | 0.08 ± 0.00 | 0.52 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
Pen333 | ΔxylB ΔaldA | pEXT20-khkC-aldoB + pACT3-yqhD | 0.11 ± 0.01 | 0.90 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.01 | 0.01 ± 0.00 |
Pen641 | ΔxylB ΔaldA | pEXT20-khkC-aldoB + pACT3-fucO | 0.16 ± 0.01 | 0.94 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.01 | 0.07 ± 0.01 |