Table 1 Ethylene glycol production with different engineered E. coli strains expressing the synthetic pathway
Experimental conditions | Final conc. (g/L) | Yielda (mol/mol) (g/g) | Productivity [g/(l h)] | References | |
|---|---|---|---|---|---|
Xylonate pathway expressed in E. coli ΔxylA mutant | [9] | ||||
Shake flask (MM + 4 g/L xylose + 1 g/L peptone + 0.5 g/L yeast extract) | 0.92 | 0.55 | 0.23 | ni | |
Bioreactor (MM + 40 g/L xylose + 10 g/L peptone + 5 g/L yeast extract, batch process) | 11.7 | 0.71 | 0.29 | 0.24 | |
(D)-ribulose-1P pathway (dte + fucA + fucO + fucK) expressed in E. coli ΔxylB ΔaldA (ΔendA ΔrecA) mutant | [10] | ||||
Shake flask (MM + 10 g/L xylose) | 3.5 | 0.84 | 0.35 | ni | |
Bioreactor (MM + unknown amount of xylose, fed-batch process) | 42.0 | ni | ni | 0.6 | |
(d)-xylulose-1P pathway (khkC + aldoB) expressed in E. coli ΔxylB mutant | [8] | ||||
Shake flask (MM + 10 g/L xylose) | 1.9 | 0.45 | 0.19 | ni | |
(d)-xylulose-1P pathway (khkC + aldoB + fucO) expressed in E. coli ΔxylB ΔaldA mutant | This study | ||||
Shake flask (MM + 10 g/L xylose) | 4.1 | 0.94 | 0.39 | ni | |
Bioreactor (MM + 55 g/L xylose + 1 g/L peptone, batch process) | 20.3 | 0.91 | 0.38 | 0.37 | |