Skip to main content

Table 3 The strains, plasmids and primers used in this study

From: Developing a high-throughput screening method for threonine overproduction based on an artificial promoter

 

Characteristics

Source

Strains

 MG1655

A substrain of E. coli strain K-12, with a relevant GenBank accession No: NC_000913.3

Lab stock

 ThrH

A threonine-producing E. coli mutant containing a plasmid-borne threonine biosynthesis operon thrABC with streptomycin resistance as selective marker

Lab stock

 ThrL

Derived from the ThrH strain by deleting the plasmid

Lab stock

Plasmids

 pET21a-egfp

An enhanced green fluorescent gene (egfp) linked to the plasmid pET21a

Lab stock

 pSB4K5-I52002

Kmr, GenBank accession NO: EU496099

[33]

 pTZL1

Kmr, constructed by cloning the cysJHp promoter and the lacZ gene into pSB4K5-I52002

This study

 pTZL2

Kmr, constructed by cloning the cysJHp promoter and the egfp gene into pSB4K5-I52002

This study

Primers

 CysJp-1

CGCCCTAGGATCCGTTGCGCAAAATCGCTGATTTATC

This study

 CysJp-2

CAAGCGACGCCAGGATTTCCGGTAAGCAAAGCTGTTTCTG

This study

 CysHp-1

CAGAAACAGCTTTGCTTACCGGAAATCCTGGCGTCGCTTG

This study

 CysHp-2

TTACCGCGCGGTGCCTTGCCTGATGCGAC

This study

 lacZP-1

TATGGCGCGCCTTTAAGAAGGAGATATACATATGACCATGATTACGGATTC

This study

 lacZP-2

GCCACTAGTTTATTTTTGACACCAGACCAACTGGTAATGGTAGCG

This study

 EgfpP-1

TATGGCGCGCCTTTAAGAAGGAGATATACATATGGTGAGCAAGGGCGAGGAGC

This study

 EgfpP-2

GCCACTAGTTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGG

This study

 B4K5P-1

GCGTACCTAGGGAATTCGAGTCACTAAGGGCTAACTAAC

This study

 B4K5P-2

GCCACTAGTAGCGGCCGCTGCAGGAGTCAC

This study