Characteristics | Source | |
---|---|---|
Strains | ||
MG1655 | A substrain of E. coli strain K-12, with a relevant GenBank accession No: NC_000913.3 | Lab stock |
ThrH | A threonine-producing E. coli mutant containing a plasmid-borne threonine biosynthesis operon thrABC with streptomycin resistance as selective marker | Lab stock |
ThrL | Derived from the ThrH strain by deleting the plasmid | Lab stock |
Plasmids | ||
pET21a-egfp | An enhanced green fluorescent gene (egfp) linked to the plasmid pET21a | Lab stock |
pSB4K5-I52002 | Kmr, GenBank accession NO: EU496099 | [33] |
pTZL1 | Kmr, constructed by cloning the cysJHp promoter and the lacZ gene into pSB4K5-I52002 | This study |
pTZL2 | Kmr, constructed by cloning the cysJHp promoter and the egfp gene into pSB4K5-I52002 | This study |
Primers | ||
CysJp-1 | CGCCCTAGGATCCGTTGCGCAAAATCGCTGATTTATC | This study |
CysJp-2 | CAAGCGACGCCAGGATTTCCGGTAAGCAAAGCTGTTTCTG | This study |
CysHp-1 | CAGAAACAGCTTTGCTTACCGGAAATCCTGGCGTCGCTTG | This study |
CysHp-2 | TTACCGCGCGGTGCCTTGCCTGATGCGAC | This study |
lacZP-1 | TATGGCGCGCCTTTAAGAAGGAGATATACATATGACCATGATTACGGATTC | This study |
lacZP-2 | GCCACTAGTTTATTTTTGACACCAGACCAACTGGTAATGGTAGCG | This study |
EgfpP-1 | TATGGCGCGCCTTTAAGAAGGAGATATACATATGGTGAGCAAGGGCGAGGAGC | This study |
EgfpP-2 | GCCACTAGTTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGG | This study |
B4K5P-1 | GCGTACCTAGGGAATTCGAGTCACTAAGGGCTAACTAAC | This study |
B4K5P-2 | GCCACTAGTAGCGGCCGCTGCAGGAGTCAC | This study |