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Table 1 Comparison of LacZ expression under the control of cysJHp in strains with different threonine-producing capacities

From: Developing a high-throughput screening method for threonine overproduction based on an artificial promoter

Strains

ThrH(pTZL1)

ThrL(pTZL1)

MG1655(pTZL1)

Specific activity of LacZ (U/mg)

97.30 ± 6.53

50.44 ± 0.64

47.71 ± 3.21

Intracellular threonine concentration (g/L)a

3.19 ± 0.17

0.10 ± 0.02

0.10 ± 0.01

Extracellular threonine concentration (g/L)b

5.83 ± 0.02

Not-detected

Not-detected

  1. aDetermined by using the LC–MS/MS method.
  2. bDetermined by using the high-pressure liquid chromatography (HPLC) method. Data are the mean and standard deviation of independent triplicates.