Table 4 Sequences of oligonucleotide primers
From: Effect of Tween 40 and DtsR1 on l-arginine overproduction in Corynebacterium crenatum
Primers | Sequence (5′–3′) | Purposes |
|---|---|---|
NCgl1221-up-F | CGCAAGCTTCAAGAAAGCCCTCGTTCCAACACTG | Amplifying the left arm of NCgl1221 |
NCgl1221-up-R | ATCAGCGTCCTAAGGAATCAAAAACGCCAAGACCAGG | |
NCgl1221-down-F | GGCGTTTTTGATTCCCTTAGGACGCTGATTACAGACG | Amplifying the right arm of NCgl1221 |
NCgl1221-down-R | GCATCTAGAGGAAGGGAGTTGAAGGTGACG | |
dtsR1-up-F | CGCAAGCTTCAGCAAGTCAGCATTAGTGGAGC | Amplifying the left arm of dtsR1 |
dtsR1-up-R | GCCGATTTACAGTGTGAAATCGTAGCGG TAGG | |
dtsR1-down-F | CCGCTACGATTTCACACTGTAAATCGGCGAATCC | Amplifying the right arm of dtsR1 |
dtsR1-down-R | GCATCTAGAGGAAGGGAGTTGAAGGTGACG | |
odhA-F | TCATTGAGGCATACCGCTCC | RP of odhA |
odhA-R | TGAAGGTACGGTCCAGATCC | |
lpdA-F | TGGGACTTAACCGTGGGCT | RP of lpdA |
lpdA-R | CACACCGTTAATCATGCGGAC | |
sucB-F | TCGTGAAGCGTCCAGTTGTC | RP of sucB |
sucB-R | GGTCCTTGATGGTGGTCAGG | |
pknG-F | GGCGGCATGGGTTGGATTT | RP of pknG |
pknG-R | GTGCCTTGGTCTTGAACGGA | |
ppp-F | CGGTATTGATCGTAGCCCTG | RP of ppp |
ppp-R | CGCGACGTAAAAAGTGCTG | |
dtsR1-F | CCTCCCATCCAACAATCGCT | RP of dtsR1 |
dtsR1-R | TAAGGAACGGTCGCGGAATC | |
zwf-F | ACTGAGATTGCCGTGGTGTT | RP of zwf |
zwf-R | AGCGGATGAGCACACCTTC | |
argB-F | GTCGCGATTTAGTTGGTTTGAT | RP of argB |
argB-R | GAGGCATCGACATTAATGATGTCT | |
16s rRNA-F | AAGAAGCACCGGCTAACTAC | Reference Gene |
16s rRNA-R | CCGGGATTTCACAGACGAC |