Fig. 5From: Overexpression of artificially fused bifunctional enzyme 4CL1–CCR: a method for production of secreted 4-hydroxycinnamaldehydes in Escherichia coli In vivo production of p-coumaraldehyde in different E. coli strains. a production of p-coumaraldehyde with strains M15, M-4CL1+M-CCR and M-4CL1–CCR; b production of p-coumaraldehyde with strains BL21, B-4CL1+CCR and B-4CL1–CCR. All strains were cultivated in LB medium with 1 mM p-coumaric acid at 37 °C for 16 h. Error bars indicate mean values ± SD from three independent experiments. Nd not detected.Back to article page